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A paper by Ian Khaw, Benjamin Croop and Kyu Young Han on Optics Express top download list

A paper entitled “Flat-field illumination for quantitative fluorescence imaging” by Dr. Kyu Young Han and his students from the Optical Nanoscopy Lab was selected as one of the top 10 downloaded articles on Optics Express for June 2018.

Uniform illumination is critical in laser-based advanced fluorescence techniques such as super-resolution fluorescence nanoscopy and single-molecule imaging where spatial resolution, stoichiometry analysis and photobleaching kinetics strongly depend on the illumination intensity. However, it has been challenging to generate a flat-top profile with TIRF (total internal reflection fluorescence) illumination which is primarily used to create a thin illumination. In this paper, we converted a Gaussian beam into a flat-top beam with a specially designed aspherical lens system through a close collaboration with Asphericon GmbH. It features (i) a long working distance to be implemented in the microscope, (ii) high spatial coherence to generate TIRF illumination, (iii) achromaticity over 400-700 nm wavelengths, and (iv) simple and compact. We believe it will be a valuable tool for quantitative single-molecule imaging and borderless high-throughput imaging.

Posted Monday, July 9, 2018

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